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vitamin B.12 defisiency

we aimed to determine the effects of vitamin B1deficiency on vitamin contents of urine, liver, and blood. In the current study, rats were divided into 3 groups (n = 5, each group): the first was freely fed a complete diet (ad lib-fed control group); the second freely fed a vitamin B
1-free diet (vitamin B1deficient group); and the third pair-fed a complete diet with the same amounts of the vitamin B1eficient group (pair-fed control group). The experimental period was for 15 days. The blood concentrations of vitamin B2PLP, vitamin B12, folic acid, and biotin were lower in the pair-fed control than in the ad lib-fed control and those of nicotinamide and
pantothenic acid were the same. We conclude that Vitamin B1  deficiency did not affect concentrations of the other B-group vitamins.
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